M00058

antibody from Boster Biological Technology

Targeting: SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00058

Provider

Boster Biological Technology

Product name

Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Sonic Hedgehog/SHH detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human Sonic Hedgehog/SHH information: Molecular Weight: 49607 MW; Subcellular Localization: Sonic hedgehog protein C-product: Secreted, extracellular space . The C-terminal peptide diffuses from the cell; Tissue Specificity: Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.

Reactivity

Human

Host

Rabbit

Antibody clone number

ABE-19

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human endometrium, using Sonic Hedgehog Antibody(M00058)SHH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SHH Antibody (M00058)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image