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LearnPA5-32594
antibody from Invitrogen Antibodies
Targeting: SHH
HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung or fetal brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Sonic Hedgehog using anti-Sonic Hedgehog Polyclonal Antibody (Product # PA5-32594) in Lung Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
