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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC4641P - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Sonic Hedgehog/Shh N-Terminus PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Sonic Hedgehog/SHH in Western blots and mouse Sonic Hedgehog/SHH in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Desert Hedgehog/Dhh (N-terminus; aa 23-198) is observed and no cross-reactivity with rmIndian Hedgehog/Ihh (N-terminus; aa 66-240), rmDesert Hedgehog/Dhh (C-terminus; aa 199-396), or rmSonic Hedgehog/Shh (C-terminus; aa 199-437) is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Antigen sequence
Q62226- Isotype
- IgG
- Antibody clone number
- 171018
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Canonical and noncanonical Hedgehog pathway in the pathogenesis of multiple myeloma.
Role for hedgehog pathway in regulating growth and function of invariant NKT cells.
Blotta S, Jakubikova J, Calimeri T, Roccaro AM, Amodio N, Azab AK, Foresta U, Mitsiades CS, Rossi M, Todoerti K, Molica S, Morabito F, Neri A, Tagliaferri P, Tassone P, Anderson KC, Munshi NC
Blood 2012 Dec 13;120(25):5002-13
Blood 2012 Dec 13;120(25):5002-13
Role for hedgehog pathway in regulating growth and function of invariant NKT cells.
Syn WK, Witek RP, Curbishley SM, Jung Y, Choi SS, Enrich B, Omenetti A, Agboola KM, Fearing CM, Tilg H, Adams DH, Diehl AM
European journal of immunology 2009 Jul;39(7):1879-92
European journal of immunology 2009 Jul;39(7):1879-92
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Sonic Hedgehog/Shh in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus PE-conjugated Monoclonal Antibody (Catalog # IC4641P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
