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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [3]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C196678 - Provider product page
- Provider
- LSBio
- Product name
- CD95 / FAS Antibody (clone 5B4) LS-C196678
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B4
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of FAS expression in transfected 293T cell line by FAS monoclonal antibody (M01), clone 5B4.Lane 1: FAS transfected lysate(37.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.
