LS-C196678

antibody from LSBio

Targeting: FAS APO-1, APT1, CD95, FAS1, TNFRSF6

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C196678

Provider

LSBio

Product name

CD95 / FAS Antibody (clone 5B4) LS-C196678

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5B4

Storage

Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot analysis of FAS expression in transfected 293T cell line by FAS monoclonal antibody (M01), clone 5B4.Lane 1: FAS transfected lysate(37.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS rabbit polyclonal antibody.