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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-14756 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-10 Monoclonal Antibody (4F8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14756 targets BCL-10 in FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
- Antibody clone number
- 4F8
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-10 using BCL-10 monoclonal antibody (Product # MA5-14756) in NIH/3T3 (1), HeLa (2), MCF-7 (3) and Jurkat (4) cell lysate.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-10 using BCL-10 monoclonal antibody (Product # MA5-14756) in NIH/3T3 (1), HeLa (2), MCF-7 (3) and Jurkat (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-10 using BCL-10 monoclonal antibody (Product # MA5-14756) in NIH/3T3 (1), HeLa (2), MCF-7 (3) and Jurkat (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-10 using BCL-10 monoclonal antibody (Product # MA5-14756) in NIH/3T3 (1), HeLa (2), MCF-7 (3) and Jurkat (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of B-cell lymphoma/leukemia 10 was achieved by transfecting Raji with BCL-10 specific siRNAs (Silencer® select Product # s531592, s531590). Western blot analysis (Fig. a) was performed using whole cell extracts (40 µg lysate) from the BCL-10 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Bcl-10 Monoclonal Antibody (4F8) (Product # MA5-14756, 1:1500) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to B-cell lymphoma/leukemia 10.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Bcl-10 Monoclonal Antibody (4F8) (Product # MA5-14756) and a 30kDa band corresponding to BCL-10 (B-cell lymphoma/leukemia 10) was observed across cell lines tested along with uncharacterized bands (*) at ~40 kDa and ~50 kDa. Whole cell extracts (40 µg lysate) of Raji (Lane 1), MCF7 (Lane 2) and HeLa (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BCL-10 monoclonal antibody (Product # MA5-14756) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BCL-10 monoclonal antibody (Product # MA5-14756) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BCL-10 monoclonal antibody (Product # MA5-14756) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of BCL-10 (B-cell lymphoma/leukemia 10) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Bcl-10 Monoclonal Antibody (4F8) (Product # MA5-14756) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control HeLa cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B) using BCL-10 monoclonal antibody (Product # MA5-14756) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using BCL-10 monoclonal antibody (Product # MA5-14756) (green) and negative control (purple).
