MA1-137

antibody from Invitrogen Antibodies

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: MA1-137 - Provider product page
Provider: Invitrogen Antibodies
Product name: BRCA1 Monoclonal Antibody (MS110)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Antibody binding site (i.e.epitope) has been mapped within the N-terminal (1-304 amino acids) of Human BRCA1.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: MS110
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20°C

BRCA1 protein structure - MA1-137 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of BRCA1 was performed on human breast carcinoma tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with a BRCA1 monoclonal antibody (Product # MA1-137) diluted 1:100 in 3% BSA-PBS (right panel) or incubated with buffer alone not containing primary antibody as a negative control (left panel), overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of BRCA1 was performed on human ovary carcinoma tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissues slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with a BRCA1 monoclonal antibody (Product # MA1-137) diluted 1:50 in 3% BSA-PBS (right panel) or incubated with buffer alone not containing primary antibody as a negative control (left panel), overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.