Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-36627 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-BRCA1 (Ser1524) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 200 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.
Kaur A, Lim JYS, Sepramaniam S, Patnaik S, Harmston N, Lee MA, Petretto E, Virshup DM, Madan B
EMBO molecular medicine 2021 Apr 9;13(4):e13349
EMBO molecular medicine 2021 Apr 9;13(4):e13349
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-BRCA1 pSer1524 using Phospho-BRCA1 pSer1524 polyclonal antibody (Product # PA5-36627) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with UV, Lane 2: NIH-3T3 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-BRCA1 (Ser1524) in Lane 1: A2780 whole cell lysate (40 µg), Lane 2: SK-OVCAR3 whole cell lysate (40 µg), Lane 3: the Lung tissue lysate of mouse (40 µg), Lane 4: H1792 whole cell lysate (40 µg), Lane 5: C6 whole cell lysate (40 µg). Samples were incubated with Phospho-BRCA1 (Ser1524) polyclonal antibody (Product # PA5-36627) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-BRCA1 (Ser1524) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-BRCA1 (Ser1524) polyclonal antibody (Product # PA5-36627) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 HR and FA pathway genes are expressed in the Wnt high compartment of the small intestine Immunohistochemical staining of sections of small intestine from C57BL/6J mice for DNA repair proteins shows that the expression of BRCA1, FANCD2 and RAD51 was high in the crypts (Wnt high compartment) whereas the villi did not show any staining. Expression of DNA repair genes correlates with the Lgr5 expression in mouse intestine: Gene expression analysis of sorted Lgr5 populations from the mouse intestine (dataset GSE149311) shows that increased expression of known Wnt target genes Lgr5 and Axin2 correlates with Mybl2 , as well as with Brca1/2 , Rad51 , and Fanc genes. The horizontal lines represent mean of replicates. Small intestinal adenomas from APC min/+ mice displayed positive nuclear immunostaining for HR and FA pathway proteins BRCA1, FANCD2, and RAD51. The bottom panels are higher magnification images of the areas marked in the top panels. PORCN inhibition reduces the expression of HR and FA pathway genes and Mybl2 in the normal mouse intestine. Total RNA was isolated from small intestines of C57BL/6J mice treated with vehicle or ETC-159 (60 mg/kg) for 3 days. Expression of DNA repair genes and Mybl2 was measured by qRT-PCR. Each data point represents an individual mouse. n = 3/group. The horizontal lines represent mean of replicates. PORCN inhibition reduces the expression of HR and FA pathway proteins in the normal mouse intestine. C57BL/6J mice were treated with veh