GTX70113

antibody from GeneTex

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot (Enhanced validation)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX70113

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70113, RRID:AB_368614

Product name

BRCA1 antibody [8F7]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

References

Submitted references

BRCA1-Dependent Translational Regulation in Breast Cancer Cells.

Dacheux E, Vincent A, Nazaret N, Combet C, Wierinckx A, Mazoyer S, Diaz JJ, Lachuer J, Venezia ND
PloS one 2013;8(6):e67313

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Epigenetic modulation of BRCA1 and BRCA2 gene expression by equol in breast cancer cell lines.

Bosviel R, Durif J, Déchelotte P, Bignon YJ, Bernard-Gallon D
The British journal of nutrition 2012 Oct;108(7):1187-93

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A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.

Turner NC, Lord CJ, Iorns E, Brough R, Swift S, Elliott R, Rayter S, Tutt AN, Ashworth A
The EMBO journal 2008 May 7;27(9):1368-77

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Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.

Lee MN, Tseng RC, Hsu HS, Chen JY, Tzao C, Ho WL, Wang YC
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 1;13(3):832-8

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BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry.

Al-Mulla F, Abdulrahman M, Varadharaj G, Akhter N, Anim JT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 May;53(5):621-9

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Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.

Bernard-Gallon DJ, Dechelotte PJ, Le Corre L, Chalabi N, Vissac-Sabatier C, Bignon YJ
Anticancer research 2004 Jan-Feb;24(1):321-4

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BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.

Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW
Oncogene 2003 Jun 12;22(24):3749-58

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Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.

Okada S, Ouchi T
The Journal of biological chemistry 2003 Jan 17;278(3):2015-20

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The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators.

Reed W, Sandstad B, Holm R, Nesland JM
International journal of surgical pathology 2003 Apr;11(2):65-74

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The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators.

Reed W, Sandstad B, Holm R, Nesland JM
International journal of surgical pathology 2003 Apr;11(2):65-74

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BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.

Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5

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BRCA1 and BRCA2 proteins are expressed in milk fat globules.

Bernard-Gallon DJ, Dechelotte P, Rio PG, Bignon YJ
International journal of cancer 1999 May 31;81(5):839-43

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Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.

Yoshikawa K, Honda K, Inamoto T, Shinohara H, Yamauchi A, Suga K, Okuyama T, Shimada T, Kodama H, Noguchi S, Gazdar AF, Yamaoka Y, Takahashi R
Clinical cancer research : an official journal of the American Association for Cancer Research 1999 Jun;5(6):1249-61

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Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation.

Bernard-Gallon DJ, Dechelotte P, Rio PG, Bignon YJ
International journal of cancer 1999 Aug 27;82(5):771-3

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

40004

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE GTX70113 diluted at 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1(GTX70113) antibody at 1:200 dilution.