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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- NB100-600C - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-600C, RRID:AB_1850841
- Product name
- Mouse Monoclonal BRCA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
The epitope recognized is found bet
ween amino acids 1600-1749- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: BRCA1 Antibody (MU) [DyLight 650] [NB100-600C] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
