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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Flow cytometry [2]
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- Product number
- NB100-600 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-600, RRID:AB_10002014
- Product name
- Mouse Monoclonal BRCA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
The epitope recognized is found bet
ween amino acids 1600-1749- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.9 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references ATM activation by ionizing radiation requires BRCA1-associated BAAT1.
Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
Aglipay JA, Martin SA, Tawara H, Lee SW, Ouchi T
The Journal of biological chemistry 2006 Apr 7;281(14):9710-8
The Journal of biological chemistry 2006 Apr 7;281(14):9710-8
Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
Okada S, Ouchi T
The Journal of biological chemistry 2003 Jan 17;278(3):2015-20
The Journal of biological chemistry 2003 Jan 17;278(3):2015-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
