MA1-16618

antibody from Invitrogen Antibodies

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-16618

Provider

Invitrogen Antibodies

Product name

BRCA1 Monoclonal Antibody (KEN)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: U2OS and MCF-7 whole cell lysates.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

KEN

Vial size

100 µL

Concentration

1.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRCA1 using a monoclonal antibody (Product # MA1-16618).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of BRCA1 in U2OS whole cell lysate using Product # MA1-16618. 1 minute ECL detection.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRCA1 in U2OS whole cell lysate. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16618). 1 minute ECL detection.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRCA1 was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against BRCA1 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. BRCA1 was detected at ~ 208 kDa using BRCA1 Mouse monoclonal antibody (Product # MA1-16618) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Mouse (Product # 31437) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to Lamin A/C (74/65 kDa). BRCA1 Antibody (Product # MA1-16618) confirms silencing of BRCA1 expression.