Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
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Validation data
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- Product number
- MA1-16618 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRCA1 Monoclonal Antibody (KEN)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: U2OS and MCF-7 whole cell lysates.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- KEN
- Vial size
- 100 µL
- Concentration
- 1.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRCA1 using a monoclonal antibody (Product # MA1-16618).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of BRCA1 in U2OS whole cell lysate using Product # MA1-16618. 1 minute ECL detection.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRCA1 in U2OS whole cell lysate. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16618). 1 minute ECL detection.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRCA1 was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against BRCA1 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. BRCA1 was detected at ~ 208 kDa using BRCA1 Mouse monoclonal antibody (Product # MA1-16618) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Mouse (Product # 31437) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to Lamin A/C (74/65 kDa). BRCA1 Antibody (Product # MA1-16618) confirms silencing of BRCA1 expression.