MA1-16619

antibody from Invitrogen Antibodies

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16619

Provider

Invitrogen Antibodies

Product name

BRCA1 Monoclonal Antibody (MU)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: MCF-7 whole cell lysates.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MU

Vial size

100 µL

Concentration

1.9 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of BRCA1 in MCF-7 whole cell lysate using Product # MA1-16619. 12 minute ECL exposure.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRCA1 using a monoclonal antibody (Product # MA1-16619).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRCA1 in MCF-7 whole cell lysate. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619). 2 minute ECL exposure.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in MCF7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in 1 x 10^6 MCF-7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.