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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-17512 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRCA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 16 μg/mL
- Storage
- -20°C
Submitted references BRCA1 Antibodies Matter.
Prediction of breast cancer risk based on flow-variant analysis of circulating peripheral blood B cells.
Yang J, Qi L, Chiang HC, Yuan B, Li R, Hu Y
International journal of biological sciences 2021;17(12):3239-3254
International journal of biological sciences 2021;17(12):3239-3254
Prediction of breast cancer risk based on flow-variant analysis of circulating peripheral blood B cells.
Syeda MM, Upadhyay K, Loke J, Pearlman A, Klugman S, Shao Y, Ostrer H
Genetics in medicine : official journal of the American College of Medical Genetics 2017 Sep;19(9):1071-1077
Genetics in medicine : official journal of the American College of Medical Genetics 2017 Sep;19(9):1071-1077
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of BRCA1 was achieved by transfecting HeLa cells with BRCA1 specific siRNA (Silencer® select Product # BRCA1 S458, BRCA1 S459). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with BRCA1 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with BRCA1 Polyclonal Antibody (Product # PA5-17512, 1:100) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2,000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Significant reduction of the specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming the specificity of the antibody to BRCA1 (Green). The Images were captured at 40X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Analysis of BRCA1 antibodies by immunoprecipitation in human cell lines. (A) BRCA1 IPs with 293T/3XFlag-hBRCA1 stably transfected cell lysate. (B) BRCA1 IPs with U2OS/3xFLAG-APEX2-fused BRCA1 cell lysate. (C) BRCA1 IPs with MDA-MB-468 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Analysis of BRCA1 antibodies by ChIP-qPCR in human cancer cells. (A) BRCA1 immunoblots with CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 (two BRCA1 inducible KO cell lines) cells with/without doxycycline treatment. (B-F) ChIP-qPCR profiles of CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 cells with/without doxycycline treatment using BRCA1(Ab1) antibody at the BRCA1 (B), ATRIP (C), EXO1 (D), MAD2L1 (E) and PPM1D (F) promoter regions. 6 mug of Ab (if concentration of Ab is provided) or 15 mul of serum (if concentration is not provided) is used. **p < 0.01, *p < 0.05, Student's t-test.
