PA1-32487

antibody from Invitrogen Antibodies

Targeting: BMI1 PCGF4, RNF51

ELISA

Antibody data

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Validation data

Product number: PA1-32487 - Provider product page
Provider: Invitrogen Antibodies
Product name: BMI-1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Goat
Isotype: IgG
Vial size: 50 µg
Concentration: 0.91 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BMI1 protein structure - PA1-32487 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of BMI-1 in 20 µg of U2OS whole cell lysate (bone osteosarcoma). Sample was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed using a BMI-1 polyclonal antibody (Product # PA1-32487) at a dilution of 1:1000 in PBS containing 1% nonfat dry milk after incubation overnight at 4ºC. The membrane was washed and reacted with a 1:20,000 dilution of IR Dye800 conjugated rabbit anti-Goat IgG (H&L) for 45 min at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot using BMI-1 Polyclonal Antibody (Product # PA1-32487) shows detection of a band at 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 µg of a U2OS whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of a rabbit anti-Goat IgG [H&L] for 45 min at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-BMI-1 Polyclonal Antibody (Product # PA1-32487) and a 40 kDa band corresponding to BMI-1 was observed across tissues tested. Whole Cell Extract (40 µg lysate) of Mouse Brain (Lane 1), Mouse Kidney (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).