Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM1930b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1930b, RRID:AB_11138459
- Product name
- BMI1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- BMI1 Antibody (Cat. #AM1930b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).
- Primary Ab dilution
- 1:100~250
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of BMI1 Antibody (Cat#AM1930b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
