LS-C288481

antibody from LSBio

Targeting: BMI1 PCGF4, RNF51

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Recommended by provider)

Antibody Data

Product number

LS-C288481

Provider

LSBio

Product name

Anti-BMI1 / PCGF4 Antibody (aa276-326) LS-C288481

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Horse, Porcine, Rabbit, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human BMI1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human BMI1 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-BMI1 ( Lot4 - left and Lot3 - right) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.