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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00000648-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000648-M02, RRID:AB_437013
- Product name
- PCGF4 monoclonal antibody (M02), clone 4E10-1C5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PCGF4.
- Antigen sequence
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECL
HSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIR
SDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSAD
AANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQ
NRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHL
RKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIA
YIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTN
AGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQ
SPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKA
SVNGSSATSSG- Isotype
- IgG
- Antibody clone number
- 4E10-1C5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with the Fbxl10/Jhdm1B histone demethylase and the Bcl6 interacting corepressor.
Sánchez C, Sánchez I, Demmers JA, Rodriguez P, Strouboulis J, Vidal M
Molecular & cellular proteomics : MCP 2007 May;6(5):820-34
Molecular & cellular proteomics : MCP 2007 May;6(5):820-34
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PCGF4 monoclonal antibody (M02), clone 4E10-1C5 Western Blot analysis of PCGF4 expression in A-549 ( Cat # L025V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PCGF4 expression in transfected 293T cell line by PCGF4 monoclonal antibody (M02), clone 4E10-1C5.Lane 1: PCGF4 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BMI1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
