PA5-29891

antibody from Invitrogen Antibodies

Targeting: BMI1 PCGF4, RNF51

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-29891 - Provider product page
Provider: Invitrogen Antibodies
Product name: BMI-1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A549, H1299, HCT116, K562, THP-1, THP-1, HL-60, C8D30, BCL-1, Raw264.7. Predicted reactivity: Mouse (97%), Rat (93%), Xenopus laevis (91%), Dog (99%), Cat (99%), Pig (100%), Chicken (97%), Chimpanzee (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BMI1 protein structure - PA5-29891 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BMI1 using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BMI1 polyclonal antibody (Product # PA5-29891) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract. B. 30 µg BCL-1 whole cell lysate/extract. C. 30 µg Raw264.7 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of BMI-1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMI-1 Polyclonal Antibody (Product # PA5-29891) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BMI-1 Polyclonal Antibody detects Bmi1 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract. B. 30 µg THP-1 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BMI-1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMI-1 Polyclonal Antibody (Product # PA5-29891) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract. B. 30 µg BCL-1 whole cell lysate/extract. C. 30 µg Raw264.7 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract. B. 30 µg HL-60 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.