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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-29891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMI-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A549, H1299, HCT116, K562, THP-1, THP-1, HL-60, C8D30, BCL-1, Raw264.7.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMI1 using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BMI1 polyclonal antibody (Product # PA5-29891) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract. B. 30 µg BCL-1 whole cell lysate/extract. C. 30 µg Raw264.7 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BMI-1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMI-1 Polyclonal Antibody (Product # PA5-29891) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects Bmi1 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract. B. 30 µg THP-1 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMI-1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMI-1 Polyclonal Antibody (Product # PA5-29891) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract. B. 30 µg BCL-1 whole cell lysate/extract. C. 30 µg Raw264.7 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects BMI1 protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract. B. 30 µg HL-60 whole cell lysate/extract.10% SDS-PAGE. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (Product # PA5-29891) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. BMI-1 Polyclonal Antibody (Product # PA5-29891) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMI-1 Polyclonal Antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 µg of BMI-1 Polyclonal Antibody (Product # PA5-29891) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by BMI-1 Polyclonal Antibody (Product # PA5-29891) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
