Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-87026 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-87026, RRID:AB_1201204
- Product name
- Rabbit Polyclonal BMI-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references BMI1-mediated histone ubiquitylation promotes DNA double-strand break repair.
Ismail IH, Andrin C, McDonald D, Hendzel MJ
The Journal of cell biology 2010 Oct 4;191(1):45-60
The Journal of cell biology 2010 Oct 4;191(1):45-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).