MAB33342

antibody from R&D Systems

Targeting: BMI1 PCGF4, RNF51

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB33342

Provider

R&D Systems

Product name

Human BMI-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human BMI-1 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P35226


Isotype

IgG

Antibody clone number

384509

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.

Wu L, Zhang D, Zhou L, Pei Y, Zhuang Y, Cui W, Chen J
EBioMedicine 2019 Mar;41:384-394

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p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs.

Chang CJ, Chao CH, Xia W, Yang JY, Xiong Y, Li CW, Yu WH, Rehman SK, Hsu JL, Lee HH, Liu M, Chen CT, Yu D, Hung MC
Nature cell biology 2011 Mar;13(3):317-23

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

BMI-1 in Human Breast Cancer Tissue. BMI-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

BMI-1 in Human Breast. BMI-1 was detected in immersion fixed paraffin-embedded sections of normal human breast using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.