MA5-17053

antibody from Invitrogen Antibodies

Targeting: CDK5 PSSALRE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17053

Provider

Invitrogen Antibodies

Product name

CDK5 Monoclonal Antibody (4E4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17053 targets CDK5 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Non-human primate, and Rat samples. The MA5-17053 immunogen is purified recombinant fragment of human CDK5 expressed in E. Coli. MA5-17053 detects CDK5 which has a predicted molecular weight of approximately 35kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4E4

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), A549 (Lane 6), Jurkat (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9). The blot was probed with CDK5 Monoclonal Antibody (Product # MA5-17053, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A band at 33kDa corresponding to CDK5 was observed across the cell lines and tissues tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK5 using CDK5 monoclonal antibody (Product # MA5-17053) in HeLa (1), K562 (2), PC-12 (3) and COS-7 (4) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK5 was performed by loading 30 ug of HEK293 control (Lane 1), HEK293- CDK5 knockout (Lane 2) edigene lysates. The blot was probed with CDK5 Monoclonal Antibody (4E4) (Product # MA5-17053, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to CDK5.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody (Product # MA5-17053) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CDK5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CDK5 Monoclonal Antibody (4E4) (Product # MA5-17053) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing predominant nuclear localization. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody (Product # MA5-17053) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 monoclonal antibody (Product # MA5-17053) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of K562 cells using CDK5 monoclonal antibody (Product # MA5-17053) (green) and negative control (purple).