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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500397 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500397, RRID:AB_2229166
- Product name
- CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6)
- Antibody type
- Monoclonal
- Description
- CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDK5
- Isotype
- IgG
- Antibody clone number
- OTI2H6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK5 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (RC200342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CDK5 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CDK5 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CDK5 mouse monoclonal antibody (TA500397).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CDK5 mouse monoclonal antibody (TA500397) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK5(RC200342).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200342 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDK5 antibody(TA500397), and then analyzed by flow cytometry.
- Validation comment
- FC
