MAB3949

antibody from Novus Biologicals

Targeting: F2RL1 GPR11, PAR2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3949

Provider

Novus Biologicals

Product name

Mouse Monoclonal PAR2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Structural insight into allosteric modulation of protease-activated receptor 2.

Cheng RKY, Fiez-Vandal C, Schlenker O, Edman K, Aggeler B, Brown DG, Brown GA, Cooke RM, Dumelin CE, Doré AS, Geschwindner S, Grebner C, Hermansson NO, Jazayeri A, Johansson P, Leong L, Prihandoko R, Rappas M, Soutter H, Snijder A, Sundström L, Tehan B, Thornton P, Troast D, Wiggin G, Zhukov A, Marshall FH, Dekker N
Nature 2017 May 4;545(7652):112-115

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of PAR2 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0102B).