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- References [9]
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- Product number
- 35-2300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAR2 Monoclonal Antibody (SAM11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SAM11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Mast Cell/Proteinase Activated Receptor 2 (PAR2) Mediated Interactions in the Pathogenesis of Discogenic Back Pain.
Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.
Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5.
Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.
Analysis of protease-activated receptor-1 and -2 in human scar formation.
The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor.
Evidence that protease activated receptor 2 expression is enhanced in human coronary atherosclerotic lesions.
Constitutive protease-activated receptor-2-mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and -2.
Richards J, Tang S, Gunsch G, Sul P, Wiet M, Flanigan DC, Khan SN, Moore S, Walter B, Purmessur D
Frontiers in cellular neuroscience 2019;13:294
Frontiers in cellular neuroscience 2019;13:294
Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.
Bagher M, Larsson-Callerfelt AK, Rosmark O, Hallgren O, Bjermer L, Westergren-Thorsson G
Cell communication and signaling : CCS 2018 Sep 15;16(1):59
Cell communication and signaling : CCS 2018 Sep 15;16(1):59
Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5.
Huang LY, Stuart C, Takeda K, D'Agnillo F, Golding B
PloS one 2016;11(8):e0160875
PloS one 2016;11(8):e0160875
Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
Sitaras N, Rivera JC, Noueihed B, Bien-Aimé M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal JS, Chemtob S
The American journal of pathology 2015 Feb;185(2):581-95
The American journal of pathology 2015 Feb;185(2):581-95
Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.
Ketabchi S, Massi D, Ficarra G, Rubino I, Franchi A, Paglierani M, Simoni A, Capodiferro S, Favia G, Maiorano E, Tarantini F, Cirino G, Santucci M
Oral diseases 2007 Jul;13(4):419-25
Oral diseases 2007 Jul;13(4):419-25
Analysis of protease-activated receptor-1 and -2 in human scar formation.
Materazzi S, Pellerito S, Di Serio C, Paglierani M, Naldini A, Ardinghi C, Carraro F, Geppetti P, Cirino G, Santucci M, Tarantini F, Massi D
The Journal of pathology 2007 Aug;212(4):440-9
The Journal of pathology 2007 Aug;212(4):440-9
The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor.
Wu S, Shin J, Zhang G, Cohen M, Franco A, Sears CL
Infection and immunity 2006 Sep;74(9):5382-90
Infection and immunity 2006 Sep;74(9):5382-90
Evidence that protease activated receptor 2 expression is enhanced in human coronary atherosclerotic lesions.
Napoli C, de Nigris F, Wallace JL, Hollenberg MD, Tajana G, De Rosa G, Sica V, Cirino G
Journal of clinical pathology 2004 May;57(5):513-6
Journal of clinical pathology 2004 May;57(5):513-6
Constitutive protease-activated receptor-2-mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and -2.
Ge L, Shenoy SK, Lefkowitz RJ, DeFea K
The Journal of biological chemistry 2004 Dec 31;279(53):55419-24
The Journal of biological chemistry 2004 Dec 31;279(53):55419-24
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- Fig. 5 Expression of PAR2 in HFL-1 cells. RTqPCR was used to confirm the expression of the serine protease receptor PAR2 on HFL-1. The relative gene expression was related to the housekeeping gene 18 s ( n = 2, 1 experiment). Human lung fibroblasts, HFL-1, showed a high gene expression for PAR2, while LAD2 cells did not show any PAR2 gene expression ( a ). Expression of PAR2 examined in monolayer cultures of HFL-1, LAD2, and in co-culture of HFL-1 and LAD2. Representative images are shown with PAR2 staining in yellow, mast cell specific tryptase-staining in red and nuclei stained with DAPI in blue ( b-m ). LAD2 cells stained by PAR2 antibodies showed no positive receptor staining ( d ), while HFL-1 fibroblasts showed a positive expression ( h ). HFL-1 showed no positive staining for tryptase ( g ), while mast cells were immunopositive ( c ). Representative scale bars are shown at the beginning of each figure ( b , f , j ) and indicated as 20 mum
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- FIGURE 4 (A) Representative images of immunohistochemical (IHC) PAR2 staining in IVD tissue from all regions; NP, AF and CEP including relevant positive and negative lung tissue controls. (B) PAR2 expression presented as % of cells expressing PAR2 as assessed by IHC and confirmed with western blot using the same PAR2 primary antibodies. Red scale bar = 100 mum, Black scale bar = 50 mum. Arrows point to same cells at both magnifications.
