MA5-34804

antibody from Invitrogen Antibodies

Targeting: METAP2 MAP2, MNPEP, p67

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34804

Provider

Invitrogen Antibodies

Product name

METAP2 Recombinant Rabbit Monoclonal Antibody (JG39-79)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Daudi, K562, mouse thymus tissue lysate, mouse kidney tissue, LOVO, PC-3M, SH-SY-5Y, rat seminal vesicle tissue, human tonsil tissue, human prostate cancer tissue, human kidney tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG39-79

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Methionine Aminopeptidase 2 in HeLa cell. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Methionine Aminopeptidase 2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Methionine Aminopeptidase 2 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Methionine Aminopeptidase 2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Methionine Aminopeptidase 2 in paraffin-embedded rat seminal vesicle tissue. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Methionine Aminopeptidase 2 in paraffin-embedded mouse testis tissue. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Methionine Aminopeptidase 2 in paraffin-embedded human tonsil tissue. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Methionine Aminopeptidase 2 in paraffin-embedded human prostate cancer tissue. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Methionine Aminopeptidase 2 in paraffin-embedded human kidney tissue. Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Methionine Aminopeptidase 2 in Daudi cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Methionine Aminopeptidase 2 monoclonal antibody (Product # MA5-34804) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.