NBP2-15501

antibody from Novus Biologicals

Targeting: ATG12 APG12, APG12L

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-15501

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ATG12 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

A novel orally available seleno-purine molecule suppresses triple-negative breast cancer cell proliferation and progression to metastasis by inducing cytostatic autophagy.

Chang CH, Bijian K, Wernic D, Su J, da Silva SD, Yu H, Qiu D, Asslan M, Alaoui-Jamali MA
Autophagy 2019 Aug;15(8):1376-1390

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MicroRNA-378 enhances migration and invasion in cervical cancer by directly targeting autophagy-related protein 12.

Tan D, Zhou C, Han S, Hou X, Kang S, Zhang Y
Molecular medicine reports 2018 May;17(5):6319-6326

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Endosomal sorting and c-Cbl targeting of paxillin to autophagosomes regulate cell-matrix adhesion turnover in human breast cancer cells.

Chang CH, Bijian K, Qiu D, Su J, Saad A, Dahabieh MS, Miller WH Jr, Alaoui-Jamali MA
Oncotarget 2017 May 9;8(19):31199-31214

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATG12 Antibody [NBP2-15501] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATG12 Antibody [NBP2-15501] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Paraffin-embedded human lung cancer. ATG12 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Paraffin-embedded rat brain. ATG12 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min