Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- AB0083-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0083-200, RRID:AB_2333112
- Product name
- Anti-ATG12
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to ATXN3. This protein appears to be a component of the ubiquitin proteasome system and has deubiquitinase activity. It may also have roles in neuroprotection, protein homeostasis maintenance, transcriptional and cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. The protein contains Qn repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease also known as spinocerebellar ataxia-3, an autosomal dominant neurologic disorder.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Dynamics of Atg5-Atg12-Atg16L1 Aggregation and Deaggregation.
Kharaziha P, Panaretakis T
Methods in enzymology 2017;587:247-255
Methods in enzymology 2017;587:247-255
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- lysate at 100 µg per lane
- Sample type
- Anti-Atg12 Ab (AB0083) at 1/500 dilution; 293HEK cells transfected with GFP-Atg12
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with methanol
- Sample type
- Hepa1-6 cell line
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated