GTX629477

antibody from GeneTex

Targeting: ATG12 APG12, APG12L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629477

Provider

GeneTex

Proper citation

GeneTex Cat#GTX629477, RRID:AB_2801283

Product name

ATG12 antibody [GT948]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µl

References

Submitted references

An estrogen antagonist, cyclofenil, has anti-dengue-virus activity.

Tohma D, Tajima S, Kato F, Sato H, Kakisaka M, Hishiki T, Kataoka M, Takeyama H, Lim CK, Aida Y, Saijo M
Archives of virology 2019 Jan;164(1):225-234

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Homologous RNA secondary structure duplications in 3' untranslated region influence subgenomic RNA production and replication of dengue virus.

Liu X, Liu Y, Zhang Q, Zhang B, Xia H, Yuan Z
Virology 2018 Nov;524:114-126

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Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.

Lai JH, Wang MY, Huang CY, Wu CH, Hung LF, Yang CY, Ke PY, Luo SF, Liu SJ, Ho LJ
EMBO reports 2018 Aug;19(8)

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Endoplasmic Reticulum Protein SCAP Inhibits Dengue Virus NS2B3 Protease by Suppressing Its K27-Linked Polyubiquitylation.

Liu H, Zhang L, Sun J, Chen W, Li S, Wang Q, Yu H, Xia Z, Jin X, Wang C
Journal of virology 2017 May 1;91(9)

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by Western blot analysis.A. 30 µg BHK21 whole cell lysate/extractB. 30 µg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells C. 30 µg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells7.5 % SDS-PAGENS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.A. 66 ?g BHK-21 whole cell extract B. 20 ?g whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 10 ?g whole cell extract of Dengue virus type 3 infected BHK-21 cells10 % SDS-PAGENS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.Blue: Hoechst 33342 staining.