Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP1151A - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1151a, RRID:AB_352576
- Product name
- ATG12 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
Ma JF, Huang Y, Chen SD, Halliday G
Neuropathology and applied neurobiology 2010 Jun;36(4):312-9
Neuropathology and applied neurobiology 2010 Jun;36(4):312-9
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of hAPG12L-T23 (Cat. #AP1151a) in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of hAPG12L-T23 (Cat. #AP1151a) in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody (Cat AP1151a) detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University."
- Primary Ab dilution
- 1:50~100