Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- ABIN2616830 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Autophagy Related 12 (ATG12) (AA 1-187) antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-187
- Isotype
- IgG
- Vial size
- 50 μg
- Storage
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for 1 year.
- Handling
- Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot of Menin antibody. Nuclear extract of HEK293 cells (20 ?g) probed with Menin antibody (1:1,000).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Menin antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 μg of chromatin from a mouse thymus and 5 μg of MEN1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify MEN1 binding sites. MEN1 is a subunit of the MLL complex which catalyzes H3K4 trimethylation and is therefore expected to localize with H3K4me3. A sampling of the MEN1 ChIP-Seq data shows the expected co-localization of MEN1 and H3K4me3.