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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C775288 - Provider product page
- Provider
- LSBio
- Product name
- APG12 / ATG12 Antibody (Atto 633) LS-C775288
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Red dye
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite.
