LS-C776840

antibody from LSBio

Targeting: ATG12 APG12, APG12L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C776840

Provider

LSBio

Product name

APG12 / ATG12 Antibody (SA) LS-C776840

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human

Host

Rabbit

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite.