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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN350095 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN350095, RRID:AB_10781046
- Product name
- anti-Autophagy Related 12 (ATG12) antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide from human ATG12, isoforms 1 and 2 (APG12, APG12L, HAPG12) conjugated to an immunogenic carrier protein has been used as the antigen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 μg
- Storage
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
- Handling
- Avoid freeze and thaw cycles.
Submitted references Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12.
Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate.
Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.
Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method.
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.
Prigione A, Cortopassi G
Autophagy 2007 Jul-Aug;3(4):377-80
Autophagy 2007 Jul-Aug;3(4):377-80
Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate.
Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T
Journal of cell science 2003 May 1;116(Pt 9):1679-88
Journal of cell science 2003 May 1;116(Pt 9):1679-88
Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.
Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E
Biochemical and biophysical research communications 2002 Sep 6;296(5):1164-70
Biochemical and biophysical research communications 2002 Sep 6;296(5):1164-70
Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method.
Mizushima N, Yoshimori T, Ohsumi Y
FEBS letters 2002 Dec 18;532(3):450-4
FEBS letters 2002 Dec 18;532(3):450-4
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.
Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E
The Journal of biological chemistry 2002 Apr 19;277(16):13739-44
The Journal of biological chemistry 2002 Apr 19;277(16):13739-44
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