TA504449

antibody from OriGene

Targeting: PDLIM5 Enh, LIM

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504449

Provider

OriGene

Proper citation

OriGene Cat#TA504449, RRID:AB_2622440

Product name

PDLIM5 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Antibody type

Monoclonal

Description

PDLIM5 mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)

Host

Mouse

Conjugate

Unconjugated

Epitope

PDLIM5

Isotype

IgG

Antibody clone number

OTI1A11

Vial size

100 µl

Concentration

3.37 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (RC200592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PDLIM5 monoclonal antibody.(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDLIM5 antibody(TA504449), and then analyzed by flow cytometry.

Validation comment

FC