PA5-38036

antibody from Invitrogen Antibodies

Targeting: NOX3 GP91-3

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-38036 - Provider product page
Provider: Invitrogen Antibodies
Product name: NOX3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA5-38036 detects Nox3 in mouse bone marrow and humanG-CSF mobilized CD34+ cells in immunofluorescence analysis. A recommended positive control is 293 cell lysate. NOX3 is not expected to cross-react with other NOX proteins.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

NOX3 protein structure - PA5-38036 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of NOX3 in human kidney tissue using a NOX3 polyclonal antibody (Product # PA5-38036) at a dilution of 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nox3 (Violet) in mouse bone marrow cells (Up). The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Cy5 (Product # A10523) at a dilution of 1:1000 for 1 hours at RT (panel b: Violet) and wash 5 times with 1xPBS before overlaid with cover slip. Nuclei (panel a: blue) were stained with DAPI (Product # D1306) during the blocking step. Panels a and b are a merged image and magnified squire area, respectively. Images were taken on a Nikon A1 confocal microscopy at 60x Objective lens with oil used. Data courtesy of Dr. Zhi Wen from the University of Wisconsin, Madison.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nox3 (Red) in human G-CSF mobilized CD34+ cells. The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Dylight 594 (Product # 35560) at a dilution of 1:1000 for 1 hours at RT (panel b: red) and wash 5 times with 1xPBS before overlaid with cover slip. Nuclei (panel a: blue) were stained with DAPI (Product # D1306) during the blocking step. Panel c was the bright field. Panels a, b and c are a merged image and magnified squire area, respectively. Images were taken on a Nikon A1 confocal microscopy at 60x Objective lens with oil used. Data courtesy of Dr. Zhi Wen from the University of Wisconsin, Madison.