PA5-25614

antibody from Invitrogen Antibodies

Targeting: SLC10A1 NTCP

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25614

Provider

Invitrogen Antibodies

Product name

NTCP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Robust in vitro assay for analyzing the neutralization activity of serum specimens against hepatitis B virus.

Zhang YL, Gao Y, Cao JL, Zhao JH, Zhang TY, Yang CL, Xiong HL, Wang YB, Ou SH, Cheng T, Chen CR, Yuan Q, Xia NS
Emerging microbes & infections 2019;8(1):724-733

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of K562 cells using a SLC10A1 polyclonal antibody (Product # PA5-25614) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Establishment and characterization of NTCP-reconstituted HepG2 cells for in vitro HBV infection. (A) A schematic diagram depicting the dox-inducible NTCP expression cassette. The NTCP-IRES-mCherry cassette was cloned into the pLenti-CMVTRE3G-eGFP (Addgene 27570) vector between the BamHI and XbaI restriction sites. (B) Flow cytometric analyses of the dox-induced mCherry expression of the different HepG2-TetOn-NTCP cell lines. The upper panel showed the percentage of mCherry+ cell population, and the lower panel showed the mean fluorescence intensity (MFI) of mCherry+ cell population of various cell lines. (C) Flow cytometric analyses of the dox-induced PreS1-peptide (FITC conjugated) binding capability of the different HepG2-TetOn-NTCP cell lines. The upper panel presented the dose-dependent comparison on the FITC/mCherry double positive percentage of different cell lines, and the lower panel presented the FITC MFI of FITC+/mCherry+ cell populations of various cell lines when binding with myr-PreS1-FITC peptide at different dosage (500, 100 and 20 nM). (D) Western blots for NTCP expression in the different HepG2-TetOn-NTCP cell lines with or without dox induction. The NTCP was detected by a commercial rabbit polyclonal antibody against NTCP (Thermo Fisher Scientific, PA525614). (E, F) Detections of HBeAg (E) and HBsAg (F) in culture supernatants of different HepG2-TetOn-NTCP cell lines following infection with HBV at 1 GE/cell (upper panel) or 100 GE/cell (lower pa