Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF4227 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CXCR7/RDC-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects CXCR7/RDC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCR5 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1α in MSCs.
Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
PloS one 2012;7(4):e34608
PloS one 2012;7(4):e34608
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1α in MSCs.
Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
Biochemical and biophysical research communications 2010 Oct 29;401(4):509-15
Biochemical and biophysical research communications 2010 Oct 29;401(4):509-15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse CXCR7/RDC-1 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CXCR7/RDC-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CXCR7/RDC-1 in Mouse Neural Progenitor Cells by Flow Cytometry. Undifferentiated mouse neural progenitor cells were stained with Sheep Anti-Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).