PA5-23192

antibody from Invitrogen Antibodies

Targeting: ACKR3 CMKOR1, CXCR7, GPR159, RDC1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA5-23192

Provider

Invitrogen Antibodies

Product name

CXCR7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCR7/RDC1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using a CXCR7/RDC1 polyclonal antibody (Product # PA5-23192) at 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCR7 in 0.5 mg/mL Jurkat lysate. Samples were incubated in CXCR7 polyclonal antibody (Product # PA5-23192). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CXCR7 in formalin-fixed paraffin-embedded tissue section of human tonsil. Samples were incubated in CXCR7 polyclonal antibody (Product # PA5-23192) using a dilution of 5 µg/mL followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CXCR7 in formalin-fixed paraffin-embedded tissue section of human tonsil. Samples were incubated in CXCR7 polyclonal antibody (Product # PA5-23192) using a dilution of 5 µg/mL followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.