PA5-27077

antibody from Invitrogen Antibodies

Targeting: ACKR3 CMKOR1, CXCR7, GPR159, RDC1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27077

Provider

Invitrogen Antibodies

Product name

CXCR7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: PC-3, U87-MG, human CXCR7 transfected Mesenchymal stem cells. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.14 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Selective Elimination of Senescent Fibroblasts by Targeting the Cell Surface Protein ACKR3.

Takaya K, Asou T, Kishi K
International journal of molecular sciences 2022 Jun 10;23(12)

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Exploration of biomarkers from a pilot weight management study for men undergoing radical prostatectomy.

Dimachkie MD, Bechtel MD, Robertson HL, Michel C, Lee EK, Sullivan DK, Chalise P, Thrasher JB, Parker WP, Godwin AK, Pathak HB, DiGiovanni J, Shivappa N, Hébert JR, Hamilton-Reeves JM
Urologic oncology 2021 Aug;39(8):495.e7-495.e15

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CXCR7 in paraformaldehyde-fixed human pancreatic cancer cell line cells using a CXCR7 polyclonal antibody (Product # PA5-27077) at a 1:50 dilution. Blue: DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Identification of ACKR3 as a novel senescent cell surface marker protein. ( A ) SA-beta-gal staining of proliferating and senescent cells. Bar = 50 um. ( B ) BrdU absorption by proliferating and senescent cells. ( C ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of senescence-related genes using cell extracts. GAPDH was used as the housekeeping gene. ( D ) Immunostaining of IL6 and ACKR3 in young (proliferating) and senescent cells. Bar = 20 um. Y: young cells. S: senescent cells. ( E ) Western blot analysis of cell extract proteins. ( F ) Western blot analysis of whole-cell lysate proteins. ( G ) Western blot analysis of cytosolic proteins. GAPDH was used as the housekeeping protein. *; p < 0.05. RQ; relative quantification. All experiments were repeated in triplicate.