Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-28739 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CXCR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-28739 targets CXCR7 in WB applications and shows reactivity with Human samples. The PA5-28739 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 45 of Human CXCR7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.59 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis.
Panneerselvam J, Jin J, Shanker M, Lauderdale J, Bates J, Wang Q, Zhao YD, Archibald SJ, Hubin TJ, Ramesh R
PloS one 2015;10(3):e0122439
PloS one 2015;10(3):e0122439
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CXCR7 using 30 µg of A) Raji (B) K562 and C) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CXCR7 polyclonal antibody (Product # PA5-28739) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CXCR7 polyclonal Antibody(Product # PA5-28739) and ~50kDa band was observed corresponding to CXCR7 was observed across the cell lines tested. Whole cell extracts (30ug lysate) (1% SDS) of K-562 (Lane 1), Raji (Lane 2), HL-60 (Lane 3), PC-3 (Lane 4) and HeLa (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..