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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB42273 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR7/RDC-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P25106- Isotype
- IgG
- Antibody clone number
- 11G8
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of MAPK Signaling by CXCR7 Leads to Enzalutamide Resistance in Prostate Cancer.
Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration.
CXCR7 promotes melanoma tumorigenesis via Src kinase signaling.
The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer.
CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells.
Ligand-specific conformational transitions and intracellular transport are required for atypical chemokine receptor 3-mediated chemokine scavenging.
Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function.
Cathepsin K cleavage of SDF-1α inhibits its chemotactic activity towards glioblastoma stem-like cells.
CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells.
The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing.
SDF-1α is a novel autocrine activator of platelets operating through its receptor CXCR4.
Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats.
COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
Ubiquitination of CXCR7 controls receptor trafficking.
CXCR7 functions as a scavenger for CXCL12 and CXCL11.
Li S, Fong KW, Gritsina G, Zhang A, Zhao JC, Kim J, Sharp A, Yuan W, Aversa C, Yang XJ, Nelson PS, Feng FY, Chinnaiyan AM, de Bono JS, Morrissey C, Rettig MB, Yu J
Cancer research 2019 May 15;79(10):2580-2592
Cancer research 2019 May 15;79(10):2580-2592
Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration.
Willems E, Dedobbeleer M, Digregorio M, Lombard A, Goffart N, Lumapat PN, Lambert J, Van den Ackerveken P, Szpakowska M, Chevigné A, Scholtes F, Rogister B
Oncogene 2019 Jan;38(1):73-87
Oncogene 2019 Jan;38(1):73-87
CXCR7 promotes melanoma tumorigenesis via Src kinase signaling.
Xu S, Tang J, Wang C, Liu J, Fu Y, Luo Y
Cell death & disease 2019 Feb 25;10(3):191
Cell death & disease 2019 Feb 25;10(3):191
The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer.
Wang M, Yang X, Wei M, Wang Z
Journal of Cancer 2018;9(21):3898-3903
Journal of Cancer 2018;9(21):3898-3903
CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells.
D'huys T, Claes S, Van Loy T, Schols D
Heliyon 2018 Mar;4(3):e00557
Heliyon 2018 Mar;4(3):e00557
Ligand-specific conformational transitions and intracellular transport are required for atypical chemokine receptor 3-mediated chemokine scavenging.
Montpas N, St-Onge G, Nama N, Rhainds D, Benredjem B, Girard M, Hickson G, Pons V, Heveker N
The Journal of biological chemistry 2018 Jan 19;293(3):893-905
The Journal of biological chemistry 2018 Jan 19;293(3):893-905
Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function.
Cheng YH, Eby JM, LaPorte HM, Volkman BF, Majetschak M
PloS one 2017;12(11):e0187949
PloS one 2017;12(11):e0187949
Cathepsin K cleavage of SDF-1α inhibits its chemotactic activity towards glioblastoma stem-like cells.
Hira VV, Verbovšek U, Breznik B, Srdič M, Novinec M, Kakar H, Wormer J, der Swaan BV, Lenarčič B, Juliano L, Mehta S, Van Noorden CJ, Lah TT
Biochimica et biophysica acta. Molecular cell research 2017 Mar;1864(3):594-603
Biochimica et biophysica acta. Molecular cell research 2017 Mar;1864(3):594-603
CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells.
Zhang M, Qiu L, Zhang Y, Xu D, Zheng JC, Jiang L
Scientific reports 2017 Aug 15;7(1):8289
Scientific reports 2017 Aug 15;7(1):8289
The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing.
Mühlethaler-Mottet A, Liberman J, Ascenção K, Flahaut M, Balmas Bourloud K, Yan P, Jauquier N, Gross N, Joseph JM
PloS one 2015;10(5):e0125616
PloS one 2015;10(5):e0125616
SDF-1α is a novel autocrine activator of platelets operating through its receptor CXCR4.
Walsh TG, Harper MT, Poole AW
Cellular signalling 2015 Jan;27(1):37-46
Cellular signalling 2015 Jan;27(1):37-46
Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
Liu Y, Carson-Walter E, Walter KA
PloS one 2014;9(8):e103938
PloS one 2014;9(8):e103938
Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats.
Walters MJ, Ebsworth K, Berahovich RD, Penfold ME, Liu SC, Al Omran R, Kioi M, Chernikova SB, Tseng D, Mulkearns-Hubert EE, Sinyuk M, Ransohoff RM, Lathia JD, Karamchandani J, Kohrt HE, Zhang P, Powers JP, Jaen JC, Schall TJ, Merchant M, Recht L, Brown JM
British journal of cancer 2014 Mar 4;110(5):1179-88
British journal of cancer 2014 Mar 4;110(5):1179-88
COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Dréan Y, Pakdel F
BMC cancer 2014 Jun 6;14:407
BMC cancer 2014 Jun 6;14:407
Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
Ding BS, Cao Z, Lis R, Nolan DJ, Guo P, Simons M, Penfold ME, Shido K, Rabbany SY, Rafii S
Nature 2014 Jan 2;505(7481):97-102
Nature 2014 Jan 2;505(7481):97-102
Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
Liberman J, Sartelet H, Flahaut M, Mühlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph JM, Gross N
PloS one 2012;7(8):e43665
PloS one 2012;7(8):e43665
Ubiquitination of CXCR7 controls receptor trafficking.
Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R
PloS one 2012;7(3):e34192
PloS one 2012;7(3):e34192
CXCR7 functions as a scavenger for CXCL12 and CXCL11.
Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
PloS one 2010 Feb 11;5(2):e9175
PloS one 2010 Feb 11;5(2):e9175
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR7/RDC-1 in Human Squamous Cell Carcinoma. CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR7/RDC-1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
