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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1979G-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Otx2 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Otx2 in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
P32243- Isotype
- IgG
- Antibody clone number
- 246826
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation.
Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.
Kuang YL, Munoz A, Nalula G, Santostefano KE, Sanghez V, Sanchez G, Terada N, Mattis AN, Iacovino M, Iribarren C, Krauss RM, Medina MW
Stem cell research 2019 May;37:101434
Stem cell research 2019 May;37:101434
Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.
Liu Q, Van Bortle K, Zhang Y, Zhao MT, Zhang JZ, Geller BS, Gruber JJ, Jiang C, Wu JC, Snyder MP
Scientific reports 2018 Aug 28;8(1):12960
Scientific reports 2018 Aug 28;8(1):12960
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Otx2 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Otx2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1979G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
