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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- GTX104507 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104507, RRID:AB_1950606
- Product name
- JAM-B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
Grivennikov SI, Wang K, Mucida D, Stewart CA, Schnabl B, Jauch D, Taniguchi K, Yu GY, Osterreicher CH, Hung KE, Datz C, Feng Y, Fearon ER, Oukka M, Tessarollo L, Coppola V, Yarovinsky F, Cheroutre H, Eckmann L, Trinchieri G, Karin M
Nature 2012 Nov 8;491(7423):254-8
Nature 2012 Nov 8;491(7423):254-8
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- JAM-B antibody detects JAM2 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g BCL-1 whole cell lysate/extract F. 30 ?g Raw264.7 whole cell lysate/extract G. 30 ?g C2C12 whole cell lysate/extract12% SDS-PAGEJAM-B antibody (GTX104507) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: A431 (GTX27909) 12% SDS PAGEGTX104507 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B(GTX104507) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse small intestine, using JAM-B(GTX104507) antibody at 1:200 dilution.(Image courtesy of Koji Taniguchi, Ph.D (Laboratory of Dr. Michael Karin, UCSD.)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B(GTX104507) antibody at 1:200 dilution.(Image courtesy of Sergei Grivennikov Ph.D (Laboratory of Dr. Michael Karin, UCSD.)
