Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB9881-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse JAM-B/VE-JAM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse JAM-B/VE-JAM in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human JAM-1, -2, or -3 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JI59
- Isotype
- IgG
- Antibody clone number
- 150005
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the α4β1-integrin.
Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
European journal of immunology 2015 Dec;45(12):3302-12
European journal of immunology 2015 Dec;45(12):3302-12
Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schön MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
Immunology 2009 Oct;128(2):196-205
Immunology 2009 Oct;128(2):196-205
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Adhesion Mediated by JAM-B/VE-JAM and Neutral-ization by Mouse JAM-B/ VE-JAM Antibody. Recombinant Mouse JAM-B/VE-JAM Fc Chimera (Catalog # 988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse JAM-B/ VE-JAM Monoclonal Antibody (Catalog # MAB9881). The ND50 is typically 0.1-0.5 µg/mL.