PA5-21576

antibody from Invitrogen Antibodies

Targeting: JAM2 C21orf43, CD322, JAM-B, JAMB, VE-JAM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21576

Provider

Invitrogen Antibodies

Product name

JAM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.

Concentration

1 mg/mL

References

Submitted references

Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination.

Redmond SA, Mei F, Eshed-Eisenbach Y, Osso LA, Leshkowitz D, Shen YA, Kay JN, Aurrand-Lions M, Lyons DA, Peles E, Chan JR
Neuron 2016 Aug 17;91(4):824-836

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JAM2/JAMB using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a JAM2/JAMB polyclonal antibody (Product # PA5-21576) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JAM2/JAMB using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a JAM2/JAMB polyclonal antibody (Product # PA5-21576) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JAM2 was performed by separating 30 µg of human tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a JAM2 Polyclonal Antibody (Product # PA5-21576) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B (Product # PA5-21576) antibody at 1:200 dilution. (Image courtesy of Sergei Grivennikov Ph. D (Laboratory of Dr. Michael Karin, UCSD.). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B (Product # PA5-21576) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.