PA5-17620

antibody from Invitrogen Antibodies

Targeting: SMC1A DXS423E, KIAA0178, SB1.8, SMC1L1, Smcb

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17620

Provider

Invitrogen Antibodies

Product name

SMC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

50 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SMC1 was achieved by transfecting MOLT-4 with SMC1 specific siRNAs (Silencer® select Product # s15751 and s15752). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the SMC1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMC1 Polyclonal Antibody (Product # PA5-17620, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMC1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SMC1 Polyclonal Antibody (Product # PA5-17620) and a 160 kDa band corresponding to SMC1 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of MOLT-4 (Lane 1) and SH-SY5Y (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).