Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-32142 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SMC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 Antibody [NBP2-32142] - Samples: Whole cell lysate (50 ug) from 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-SMC1 antibody NBP2-32142 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total SMC1 using affinity purified rabbit anti-SMC1 antibody at 0.1 ug/ml with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMC1 Antibody [NBP2-32142] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies: SMC1 was immunoprecipitated using affinity purified rabbit anti-SMC1 antibody NBP2-32142 at 6 ug/mg lysate. Total SMC1 was immunoprecipitated using rabbit anti-SMC1 antibody at 6 ug/mg lysate. SMC1 was blotted at 0.1 ug/ml (upper panel). Total SMC1 was blotted using at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMC1 Antibody [NBP2-32142] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S957) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMC1 Antibody [NBP2-32142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S957) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).