Explore
Validate
LearnMP02148
antibody from Boster Biological Technology
Targeting: SMC1A
DXS423E, KIAA0178, SB1.8, SMC1L1, Smcb
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MP02148 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-SMC1 (S957) Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Rabbit IgG Monoclonal antibody for Q14683 detection. Tested positive for WB IHC IF/IF in Human
- Reactivity
- Human
- Host
- Rabbit
- Antibody clone number
- FCI-19
- Vial size
- 100ug
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of SMC1A using anti-SMC1A antibody (MP02148).SMC1A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMC1A Antibody (MP02148) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
