Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-204 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-204, RRID:AB_10081001
- Product name
- Rabbit Polyclonal SMC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Olaparib.
Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation.
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity.
Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice.
Loss of ATM kinase activity leads to embryonic lethality in mice.
Homozygous deficiency of ubiquitin-ligase ring-finger protein RNF168 mimics the radiosensitivity syndrome of ataxia-telangiectasia.
ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control.
The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion.
ATM is down-regulated by N-Myc-regulated microRNA-421.
Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
Roles of human AND-1 in chromosome transactions in S phase.
Rad50S alleles of the Mre11 complex: questions answered and questions raised.
Atm-haploinsufficiency enhances susceptibility to carcinogen-induced mammary tumors.
Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation.
Methylator-induced, mismatch repair-dependent G2 arrest is activated through Chk1 and Chk2.
The monofunctional alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine triggers apoptosis through p53-dependent and -independent pathways.
SMC1 involvement in fragile site expression.
MRE11 mutations and impaired ATM-dependent responses in an Italian family with ataxia-telangiectasia-like disorder.
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
The ATM-SMC1 pathway is essential for activation of the chromium[VI]-induced S-phase checkpoint.
Chromatin association of rad17 is required for an ataxia telangiectasia and rad-related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation.
Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation.
Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species.
Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
A DNA damage checkpoint response in telomere-initiated senescence.
Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts.
MDC1 is required for the intra-S-phase DNA damage checkpoint.
MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation.
53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage.
SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint.
Wang C, Jette N, Moussienko D, Bebb DG, Lees-Miller SP
Translational oncology 2017 Apr;10(2):190-196
Translational oncology 2017 Apr;10(2):190-196
Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation.
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD
The EMBO journal 2015 May 12;34(10):1399-416
The EMBO journal 2015 May 12;34(10):1399-416
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
Houlihan SL, Feng Y
eLife 2014 Sep 23;3:e03297
eLife 2014 Sep 23;3:e03297
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity.
Nakamura K, Fike F, Haghayegh S, Saunders-Pullman R, Dawson AJ, Dörk T, Gatti RA
Molecular genetics & genomic medicine 2014 Jul;2(4):332-40
Molecular genetics & genomic medicine 2014 Jul;2(4):332-40
Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice.
Puccini J, Shalini S, Voss AK, Gatei M, Wilson CH, Hiwase DK, Lavin MF, Dorstyn L, Kumar S
Proceedings of the National Academy of Sciences of the United States of America 2013 Dec 3;110(49):19920-5
Proceedings of the National Academy of Sciences of the United States of America 2013 Dec 3;110(49):19920-5
Loss of ATM kinase activity leads to embryonic lethality in mice.
Daniel JA, Pellegrini M, Lee BS, Guo Z, Filsuf D, Belkina NV, You Z, Paull TT, Sleckman BP, Feigenbaum L, Nussenzweig A
The Journal of cell biology 2012 Aug 6;198(3):295-304
The Journal of cell biology 2012 Aug 6;198(3):295-304
Homozygous deficiency of ubiquitin-ligase ring-finger protein RNF168 mimics the radiosensitivity syndrome of ataxia-telangiectasia.
Devgan SS, Sanal O, Doil C, Nakamura K, Nahas SA, Pettijohn K, Bartek J, Lukas C, Lukas J, Gatti RA
Cell death and differentiation 2011 Sep;18(9):1500-6
Cell death and differentiation 2011 Sep;18(9):1500-6
ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control.
Gatei M, Jakob B, Chen P, Kijas AW, Becherel OJ, Gueven N, Birrell G, Lee JH, Paull TT, Lerenthal Y, Fazry S, Taucher-Scholz G, Kalb R, Schindler D, Waltes R, Dörk T, Lavin MF
The Journal of biological chemistry 2011 Sep 9;286(36):31542-56
The Journal of biological chemistry 2011 Sep 9;286(36):31542-56
The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion.
Rajesh C, Baker DK, Pierce AJ, Pittman DL
Nucleic acids research 2011 Jan;39(1):132-45
Nucleic acids research 2011 Jan;39(1):132-45
ATM is down-regulated by N-Myc-regulated microRNA-421.
Hu H, Du L, Nagabayashi G, Seeger RC, Gatti RA
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1506-11
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1506-11
Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
Waltes R, Kalb R, Gatei M, Kijas AW, Stumm M, Sobeck A, Wieland B, Varon R, Lerenthal Y, Lavin MF, Schindler D, Dörk T
American journal of human genetics 2009 May;84(5):605-16
American journal of human genetics 2009 May;84(5):605-16
Roles of human AND-1 in chromosome transactions in S phase.
Yoshizawa-Sugata N, Masai H
The Journal of biological chemistry 2009 Jul 31;284(31):20718-28
The Journal of biological chemistry 2009 Jul 31;284(31):20718-28
Rad50S alleles of the Mre11 complex: questions answered and questions raised.
Usui T, Petrini JH, Morales M
Experimental cell research 2006 Aug 15;312(14):2694-9
Experimental cell research 2006 Aug 15;312(14):2694-9
Atm-haploinsufficiency enhances susceptibility to carcinogen-induced mammary tumors.
Lu S, Shen K, Wang Y, Santner SJ, Chen J, Brooks SC, Wang YA
Carcinogenesis 2006 Apr;27(4):848-55
Carcinogenesis 2006 Apr;27(4):848-55
Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation.
Escobar J, Frank JW, Suryawan A, Nguyen HV, Kimball SR, Jefferson LS, Davis TA
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E914-21
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E914-21
Methylator-induced, mismatch repair-dependent G2 arrest is activated through Chk1 and Chk2.
Adamson AW, Beardsley DI, Kim WJ, Gao Y, Baskaran R, Brown KD
Molecular biology of the cell 2005 Mar;16(3):1513-26
Molecular biology of the cell 2005 Mar;16(3):1513-26
The monofunctional alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine triggers apoptosis through p53-dependent and -independent pathways.
Kim WJ, Beardsley DI, Adamson AW, Brown KD
Toxicology and applied pharmacology 2005 Jan 1;202(1):84-98
Toxicology and applied pharmacology 2005 Jan 1;202(1):84-98
SMC1 involvement in fragile site expression.
Musio A, Montagna C, Mariani T, Tilenni M, Focarelli ML, Brait L, Indino E, Benedetti PA, Chessa L, Albertini A, Ried T, Vezzoni P
Human molecular genetics 2005 Feb 15;14(4):525-33
Human molecular genetics 2005 Feb 15;14(4):525-33
MRE11 mutations and impaired ATM-dependent responses in an Italian family with ataxia-telangiectasia-like disorder.
Delia D, Piane M, Buscemi G, Savio C, Palmeri S, Lulli P, Carlessi L, Fontanella E, Chessa L
Human molecular genetics 2004 Sep 15;13(18):2155-63
Human molecular genetics 2004 Sep 15;13(18):2155-63
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
Takahashi TS, Yiu P, Chou MF, Gygi S, Walter JC
Nature cell biology 2004 Oct;6(10):991-6
Nature cell biology 2004 Oct;6(10):991-6
The ATM-SMC1 pathway is essential for activation of the chromium[VI]-induced S-phase checkpoint.
Wakeman TP, Kim WJ, Callens S, Chiu A, Brown KD, Xu B
Mutation research 2004 Oct 4;554(1-2):241-51
Mutation research 2004 Oct 4;554(1-2):241-51
Chromatin association of rad17 is required for an ataxia telangiectasia and rad-related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation.
Garg R, Callens S, Lim DS, Canman CE, Kastan MB, Xu B
Molecular cancer research : MCR 2004 Jun;2(6):362-9
Molecular cancer research : MCR 2004 Jun;2(6):362-9
Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation.
Jowsey PA, Doherty AJ, Rouse J
The Journal of biological chemistry 2004 Dec 31;279(53):55562-9
The Journal of biological chemistry 2004 Dec 31;279(53):55562-9
Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species.
Kurz EU, Douglas P, Lees-Miller SP
The Journal of biological chemistry 2004 Dec 17;279(51):53272-81
The Journal of biological chemistry 2004 Dec 17;279(51):53272-81
Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.
Hickson I, Zhao Y, Richardson CJ, Green SJ, Martin NM, Orr AI, Reaper PM, Jackson SP, Curtin NJ, Smith GC
Cancer research 2004 Dec 15;64(24):9152-9
Cancer research 2004 Dec 15;64(24):9152-9
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Udayakumar D, Bladen CL, Hudson FZ, Dynan WS
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
A DNA damage checkpoint response in telomere-initiated senescence.
d'Adda di Fagagna F, Reaper PM, Clay-Farrace L, Fiegler H, Carr P, Von Zglinicki T, Saretzki G, Carter NP, Jackson SP
Nature 2003 Nov 13;426(6963):194-8
Nature 2003 Nov 13;426(6963):194-8
Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts.
Musio A, Montagna C, Zambroni D, Indino E, Barbieri O, Citti L, Villa A, Ried T, Vezzoni P
Cancer research 2003 Jun 1;63(11):2855-63
Cancer research 2003 Jun 1;63(11):2855-63
MDC1 is required for the intra-S-phase DNA damage checkpoint.
Goldberg M, Stucki M, Falck J, D'Amours D, Rahman D, Pappin D, Bartek J, Jackson SP
Nature 2003 Feb 27;421(6926):952-6
Nature 2003 Feb 27;421(6926):952-6
MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation.
Wang Y, Qin J
Proceedings of the National Academy of Sciences of the United States of America 2003 Dec 23;100(26):15387-92
Proceedings of the National Academy of Sciences of the United States of America 2003 Dec 23;100(26):15387-92
53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage.
Mochan TA, Venere M, DiTullio RA Jr, Halazonetis TD
Cancer research 2003 Dec 15;63(24):8586-91
Cancer research 2003 Dec 15;63(24):8586-91
SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint.
Yazdi PT, Wang Y, Zhao S, Patel N, Lee EY, Qin J
Genes & development 2002 Mar 1;16(5):571-82
Genes & development 2002 Mar 1;16(5):571-82
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 Antibody [NB100-204] - Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC1 Antibody [NB100-204] - Detection of human SMC1 in HeLa nuclear extracts (50 ug), Xenopus oocytes (100 ug), and MEF whole cell lysate (100 ug) in Western blot using NB 100-204.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMC1 Antibody [NB100-204] - Detection of Human SMC1 by Western Blot of Immunoprecipitates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
