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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- NBP1-47257 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47257, RRID:AB_10010211
- Product name
- Rabbit Polyclonal DNA polymerase alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references KSHV-encoded LANA protects the cellular replication machinery from hypoxia induced degradation.
Singh RK, Lamplugh ZL, Lang F, Yuan Y, Lieberman P, You J, Robertson ES
PLoS pathogens 2019 Sep;15(9):e1008025
PLoS pathogens 2019 Sep;15(9):e1008025
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody NBP1-47257 used for WB at 0.04 ug/mL (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47256, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.
